PEG-MGF: research overview
What is PEG-MGF
PEG-MGF is a pegylated form of the MGF peptide, also known as mechano growth factor. MGF arises from alternative splicing of the IGF-1 gene transcript, which gives it a C-terminal end that differs from classic IGF-1. Polyethylene glycol (PEG) chains are attached to the peptide. The purpose of this modification is longer stability of the peptide in solution.
You can buy PEG-MGF as a research use only reagent in a 2 mg lyophilized vial. Every batch is checked for purity and mass.
Molecular structure
The molecule has two elements:
- MGF peptide core, a splice variant of the IGF-1 transcript with a unique C-terminal end.
- PEG chains, polyethylene glycol polymer attached to the peptide.
Attaching PEG increases the molecular mass and changes how the molecule behaves in solution. In the lab, this means a different stability profile from an unmodified peptide, so it is worth factoring into your experimental design.
Mechanism described in research
In vitro studies on muscle satellite cell cultures have described the effect of MGF on the activation and proliferation of these cells. Animal models have shown MGF involvement in the regeneration of muscle tissue after injury. The pegylated form lets researchers study the same pathway with longer peptide stability in solution.
Research areas
- Biology of muscle satellite cells in in vitro cultures
- Muscle tissue regeneration in animal models
- The GH/IGF-1 axis and its splicing variants
- Stability of pegylated peptides in solutions
- Comparisons with IGF-1 and other peptides of the growth hormone axis
Product profile
| Parameter | Value |
|---|---|
| Name | PEG-MGF |
| Category | GH axis |
| Form | Lyophilized powder |
| Purity | 99% or higher (HPLC) |
| Molecular mass | Confirmed by MS |
| Available sizes | 2 mg |
Working in the lab
Storage
Keep the lyophilized powder at -20 °C, tightly sealed and away from moisture. Once reconstituted, store the solution at 2-8 °C. Avoid repeated freezing and thawing, because each cycle can reduce the quality of the solution.
Dissolving and reconstitution
Water is the most commonly used solvent. In peptide work, bacteriostatic water is also common because it contains a preservative that limits microbial growth. Dilute acetic acid is used when a protocol calls for better solubility of the peptide. Your research protocol sets the volume and concentration, and our concentration calculator converts the weight of the powder into solution concentration.
Batch quality
Every batch is tested by HPLC, which confirms purity of 99% or higher. Mass spectrometry (MS) confirms the molecular mass. You can read how we control quality on our quality page.
Related products
- MGF: the unpegylated form of the same peptide, for comparisons in research.
- IGF-1 LR3: an IGF-1 analogue for research on the GH/IGF-1 axis.
- CJC-1295 no DAC: a secretagogue for research on the growth hormone axis.
You will find the full list of reagents in the GH axis category, and you can convert concentrations in the concentration calculator.
